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991.
992.
Long Jin Hai-Ying Zhu Qing Guo Xiao-Chen Li Yu-Chen Zhang Guang-Lei Zhang Xiao-Xu Xing Mei-Fu Xuan Qi-Rong Luo Xi-Jun Yin Jin-Dan Kang 《Biotechnology letters》2016,38(9):1433-1441
Objective
To examine the effect of PCI-24781 (abexinostat) on the blastocyst formation rate in pig somatic cell nuclear transferred (SCNT) embryos and acetylation levels of the histone H3 lysine 9 and histone H4 lysine 12.Results
Treatment with 0.5 nM PCI-24781 for 6 h significantly improved the development of cloned embryos, in comparison to the control group (25.3 vs. 10.5 %, P < 0.05). Furthermore, PCI-24781 treatment led to elevated acetylation of H3K9 and H4K12. TUNEL assay and Hoechst 33342 staining revealed that the percentage of apoptotic cells in blastocysts was significantly lower in PCI-24781-treated SCNT embryos than in untreated embryos. Also, PCI-24781-treated embryos were transferred into three surrogate sows, one of whom became pregnant and two fetuses developed.Conclusion
PCI-24781 improves nuclear reprogramming and the developmental potential of pig SCNT embryos.993.
Jun Feng Su Sheng Chen Zheng Ting Lin Huang Fang Ma Si Cheng Shao Shao Fei Yang 《Geomicrobiology journal》2016,33(7):586-591
A novel bacterium, strain SZ28, identified as Acinetobacter sp., showed anaerobic denitrification ability using Mn(II) as the electron donor. Nitrate-nitrogen concentration decreased from nearly 16.52–mg L?1 to 4.4–mg L?1, without accumulation of nitrite as an intermediate, with a maximum of 0.063–mg NO3?-N L?1 h?1, reaching a peak of 0.085–mg NO3?-N L?1 h?1 in sodium acetate. The nitrate removal rate reached 0.067–mg NO3?-N L?1 h?1, 0.059–mg NO3?-N L?1 h?1, and 0.078 mg NO3?-N L?1 h?1 using Mn(II), S(II), and Fe(II) as electron donors, respectively. The optimum pH was 6.0, with a removal rate of 0.063–mg NO3?-N L?1 h?1 相似文献
994.
995.
996.
Distribution and budget of dissolved and biogenic silica in the Bohai Sea and Yellow Sea 总被引:2,自引:0,他引:2
Jun Liu Jiaye Zang Lex Bouwman Sen Liu Zhigang Yu Xiangbin Ran 《Biogeochemistry》2016,130(1-2):85-101
997.
998.
Chen Jun Gu Siyang Hao Honghong Chen Jianmeng 《Applied microbiology and biotechnology》2016,100(22):9787-9794
Applied Microbiology and Biotechnology - A novel heterotrophic nitrification-aerobic denitrification bacterium, Alcaligenes sp. TB (GenBank accession no. JQ044686), was isolated from a rotating... 相似文献
999.
Hao Jiang Changhong Duan Mingfang Luo Xin-Hui Xing 《Applied microbiology and biotechnology》2016,100(24):10331-10341
In methane-rich environments, methane-oxidizing bacteria usually occur predominantly among consortia including other types of microorganisms. In this study, artificial coal bed gas and methane gas were used to enrich mixed methanotrophic cultures from the soil of a coal mine in China, respectively. The changes in microbial community structure and function during the enrichment were examined. The microbial diversity was reduced as the enrichment proceeded, while the capacity for methane oxidation was significantly enhanced by the increased abundance of methanotrophs. The proportion of type II methanotrophs increased greatly from 7.84 % in the sampled soil to about 50 % in the enrichment cultures, due to the increase of methane concentration. After the microbial community of the cultures got stable, Methylomonas and Methylocystis became the dominant type I and type II methanotrophs, while Methylophilus was the prevailing methylotroph. The sequences affiliated with pigment-producing strains, Methylomonas rubra, Hydrogenophaga sp. AH-24, and Flavobacterium cucumis, could explain the orange appearance of the cultures. Comparing the two cultures, the multi-carbon sources in the artificial coal bed gas caused more variety of non-methanotrophic bacteria, but did not help to maintain the diversity or to increase the quantity and activity of methanotrophs. The results could help to understand the succession and interaction of microbial community in a methane-driven ecosystem. 相似文献
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